ABSTRACT
The addition of hemoglobin [Hb] caused an inhibitory effect on the luminol-dependent chemiluminesence [CL] induced by the excitation of luminol by the oxidative metabolic, hydrogen peroxide, In a cell free medium. The CL was detected with an ultra-high sensitive photon counting system designed and built in the department of physiology. The Inhibitory effect produced by various Hb levels was dose dependent, reproducible and linear with an r=0.997. Hb concentration curves constructed by CL and standard Cyanmethaemoglobin [HiCN] methods were parallel. A comparison between the inhibited CL [area under the curves], and the optical density [HiCN method] produced by same Hb levels was linear with an r=0.990. There was no significant difference [0.1 > P < 0.5] between Rb level measured by CL and HICN method in healthy adults samples. A point of importance, turbidity due to high leukocytes count [250 x 109 CIL] has no significant effect on Hb levels measured by CL and the modified HICN methods. These results suggest that, CL method may provide an additional reliable method for Hb estimation